Self-powered Microfluidic Origami Electrochemiluminescence Biosensing Platform

1 Reagents and instruments FeCl3, anhydrous AlCl3, β-D-glucose, maltose, R-lactose and D-fructose were obtained from Beijing Chemical Reagent Company (Beijing, China), and the carbohydrate solutions were left at room temperature for 24 h before use. Al foil tape and conductive carbon ink were purchased commercially. Ru(bpy)3Cl6·6H2O, Luminol and glucose oxidase (from Aspergillus niger, GOx) were purchased from Sigma-Aldrich (Milwaukee, WI). All other chemicals used were of analytical reagent grade without any further purification. Double distilled water was used throughout. Electrochemical measurements were conducted with an electrochemical workstation CHI 832B (Shanghai Chenhua Instrument Corporation, China). ECL signals were obtained by a model MPI-A capillary electrophoresis ECL system (Xi’an Remax Electronics Co. Ltd.). All of the experiments were carried out at room temperature.